Publication database

FemtoLux 3 laser was used as an illumination source in the wide-field second harmonic generation (SHG) microscope. Relatively high laser pulse energy at a medium pulse repetition frequency allowed for a faster single image acquisition compared to that using laser-scanning. It was also possible to acquire images of relatively large sample areas, which paved the way for the rapid imaging of macroscopic sample areas with microscopic resolution.

Photoacoustic microscopy (PAM) is an emerging imaging technology that can non-invasively visualize ocular structures in animal eyes. This report describes an integrated multimodality imaging system that combines PAM, optical coherence tomography (OCT), and fluorescence microscopy (FM) to evaluate angiogenesis in larger animal eyes. High-resolution in vivo imaging was performed in live rabbit eyes with vascular endothelial growth factor (VEGF)-induced retinal neovascularization (RNV). The results demonstrate that our multimodality imaging system can non-invasively visualize RNV in both albino and pigmented rabbits to determine retinal pathology using PAM and OCT and verify the leakage of neovascularization using FM and fluorescein dye. This work presents high-resolution visualization of angiogenesis in rabbits using a multimodality PAM, OCT, and FM system and may represent a major step toward the clinical translation of the technology.